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integrin α6 subunit f6  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology integrin α6 subunit f6
    (A) SIMS cells harboring an inducible shRNA targeting the <t>integrin</t> <t>α6</t> subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.
    Integrin α6 Subunit F6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/integrin+%CE%B16+subunit+f6/bio_rxiv__830562-108-23-36?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 173 article reviews
    integrin α6 subunit f6 - by Bioz Stars, 2026-07
    93/100 stars

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    1) Product Images from "Alpha 6 integrins promote cytokinesis by regulating the expression of RSK2 and MKLP1"

    Article Title: Alpha 6 integrins promote cytokinesis by regulating the expression of RSK2 and MKLP1

    Journal: bioRxiv

    doi: 10.1101/830562

    (A) SIMS cells harboring an inducible shRNA targeting the integrin α6 subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.
    Figure Legend Snippet: (A) SIMS cells harboring an inducible shRNA targeting the integrin α6 subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.

    Techniques Used: shRNA, Expressing, Knockdown, Western Blot, Control, Staining, Quantitation Assay, Transfection



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    integrin α6 subunit f6  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology integrin α6 subunit f6
    (A) SIMS cells harboring an inducible shRNA targeting the <t>integrin</t> <t>α6</t> subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.
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    mouse monoclonal antibody to the integrin α6 subunit (f6)  (Santa Cruz Biotechnology)
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    (A) SIMS cells harboring an inducible shRNA targeting the <t>integrin</t> <t>α6</t> subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.
    Mouse Monoclonal Antibody To The Integrin α6 Subunit (F6), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) SIMS cells harboring an inducible shRNA targeting the integrin α6 subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.

    Journal: bioRxiv

    Article Title: Alpha 6 integrins promote cytokinesis by regulating the expression of RSK2 and MKLP1

    doi: 10.1101/830562

    Figure Lengend Snippet: (A) SIMS cells harboring an inducible shRNA targeting the integrin α6 subunit were treated with doxycycline to induce the expression of the α6 shRNA or left untreated. The efficiency of α6 knockdown was assayed by western blot. Shown is a representative blot probed with antibodies to the α6 integrin subunit and reprobed with antibodies to GAPDH as a loading control. (B) Cells expressing the shRNA or control cells were stained for α-tubulin and DNA to quantify cells connected by midbodies and (C) binucleated cells. Three hundred individual cells were analyzed from each of three independent experiments performed in triplicate. Plotted is the mean percentage of cells with mibodies or that are binucleated ± s.d. (n = 9). (D) Effect of α6 depletion by shRNA on the expression of midbody proteins required for cytokinesis was assayed by western blot. Shown is a representative blot. (E) Quantitation from three independent experiments ± s.d. (F) The effect of α6 depletion by shRNA on mRNA expression was assayed by qPCR. Data is shown from two independent experiments ± variance. (G-L) SIMS cells were transfected with either NT or α6 targeting siRNA. The effect of α6 depletion on cytokinesis (G-I) and midbody components was assayed as above for α6 targeting shRNA (K-L). (K) Shown is a representative blot and (L) quantitation of protein expression from three independent experiments. **p<0.05, *p<0.001.

    Article Snippet: Mouse monoclonal antibody to α-tubulin (DM1α), goat polyclonal antibody to RSK2 (C-19), rabbit polyclonal antibody to CEP55 (M-300), mouse monoclonal antibody to the integrin α6 subunit (F6), and rabbit polyclonal antibodies to RSK1 (C21) were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).

    Techniques: shRNA, Expressing, Knockdown, Western Blot, Control, Staining, Quantitation Assay, Transfection